Fig. 7

TDRKH-AS1 elevates the DDIT4 expression induced pyroptosis through PDIA4 binding. A TDRKH-AS1, DDIT4 and PDIA4 were upregulated in HUVECs with TDRKH-AS1 overexpression. B, C The expression of DDIT4 on mRNA and protein levels were increased by TDRKH-AS1 or PDIA4 overexpression, while the opposite results were obtained with TDRKH-AS1 or PDIA4 knockdown. D, E The interaction between PDIA4 and DDIT4 by Co-IP analyses and western blotting in HUVECs. F TDRKH-AS1 or PDIA4 overexpression increased the expression of pyroptosis-associated proteins and partially blocked with knockdown of DDIT4 using western blotting in HUVECs. G–I Knockdown of DDIT4 partially attenuated LDH, IL-1β and IL-18 release induced by overexpression TDRKH-AS1 or PDIA4 in HUVECs by LDH activity assay and ELISA. The data are represented as the mean ± SD; **p < 0.01, *p < 0.05