Fig. 6

Fgr interacted with SIRT1 and affected its activity. A, B Co-immunoprecipitation assay indicating that Fgr interacts with SIRT1 in the hippocampus of WT mice in vivo. C–F Representative Western blot and statistical analysis of Fgr and SIRT1 in HT22 mouse hippocampal neuronal cell line (n = 3, mean ± SEM, unpaired t-test, *p < 0.05). G SIRT1 activity in the hippocampus of septic mice (n = 3 or 4 mice, mean ± SEM, one-way ANOVA with Bonferroni post hoc test, *p < 0.05, **p < 0.01). H SIRT1 activity in HT22 cells (n = 4, mean ± SEM, one-way ANOVA with Bonferroni post hoc test, ***p < 0.001). I Schematic representation of mouse SIRT1 fragments. J Fgr enzymatic activity measured with a Universal Kinase Activity Kit and Shp2 as a substrate (n = 3 or 6, mean ± SEM, one-way ANOVA with Bonferroni post hoc test, *p < 0.05, **p < 0.01)