Fig. 3
From: Attenuated Dengue virus PV001-DV induces oncolytic tumor cell death and potent immune responses
PBMCs treated with PV001-DV produce cytotoxic soluble mediators. A, top Schematic of melanoma and breast cancer patient PBMCs incubated with PV001-DV (DV) for 3Â days. Cells well removed by centrifugation and virus was removed by filtration. A, bottom Schematic of resultant supernatant incubated with A2058 and SK-MEL5 melanoma or MDA-MB-231 breast cancer cells. B Photomicrograph of cells at 10X magnification from (A) at 1Â day of incubation. C Viability of melanoma cells measured with Aqua Live/Dead from experiment in A after incubation with melanoma patient PBMC-derived supernatant. D Viability of breast cancer and melanoma cells measured with Aqua Live/Dead from experiment in A after incubation with breast cancer patient PBMC-derived supernatant