Fig. 4

Involvement of mitochondrial metabolism in IL-1RA-promoted malignant behaviors of OSCC cells. A–B OECM-1 cells with IL-1RA overexpression and control cells were assessed for tumorsphere formation in the presence or absence of rotenone or antimycin A in (A), and metformin or phenformin in (B). C–D OECM-1 cells with IL-1RA overexpression and control cells were assessed for transwell cell migration ability in the presence or absence of rotenone or antimycin A in (C), and metformin or phenformin in (D). E OECM-1 cells with IL-1RA overexpression and control cells were assessed for transwell cell invasion ability in the presence or absence of metformin or phenformin. Data were presented as mean ± SD from three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001. EV, empty vector; IL-1RA-OE, overexpression of IL-1RA