Fig. 2

Effect of IL-1RA expression on in vitro cell growth of OSCC cells. A–F Following knockdown of IL-1RA expression in HSC-3 and Ca9-22 cells and overexpression of IL-1RA in OECM-1 cells, assessments were performed for clonogenic survival (A, B), tumorsphere formation (C, D), and colony formation in soft agar (E). In (F), clonogenic survival was assessed in OECM-1 cells with overexpression of IL-1RA and control cells in the presence or absence of ionizing radiation treatment. Data were presented as mean ± SD from three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001. shLuc, knockdown of firefly luciferase; shIL-1RA, knockdown of IL-1RA; EV, empty vector; IL-1RA-OE, overexpression of IL-1RA