Fig. 6

NLRP3 A, IL-1β B, caspase 1 C, and gasdermin D were quantified by western blotting in PBMCs of the study population. White bars with dots represent lean children (n = 8), grey bars represent OBIR- children (n = 8), white bars represent EP-OBIR + children (n = 12), and black bars represent LP-OBIR + children (n = 14). Values are means ± SEM. P < 0.05 was considered for statistical significance. The panel on the right are representative images of the western-blots performed for quantification. (*) shows significant differences relative to lean children, a shows significant differences relative to EP-OBIR + children, and b shows significant differences relative to OBIR- children. A.U., arbitrary units; NLRP3, NOD like receptor 3; IL-1β, interleukin 1β; PBMCs, peripheral blood mononuclear cells; ObIR-, non-insulin resistant children with obesity; ObIR + , insulin resistant children with obesity; EP-OBIR + , early peak OBIR + children; LP-OBIR + , late peak OBIR + children