Fig. 1

Levels of reactive oxygen species in erythrocytes in the study population. White bars with dots represent lean children (n = 15), grey bars represent OBIR- children (n = 10), grey bars with dots represent OBIR + children (n = 47), white bars represent EP-OBIR + children (n = 10), and black bars represent LP-OBIR + children (n = 26). A shows ROS levels in lean, OBIR- and OBIR+ groups of children. Representative histograms for lean, ObIR-, and ObIR + groups are represented in B, C and D, respectively (red represents negative control, and blue DCFDA labelled cells). E shows ROS levels in these same groups, when the ObIR + group is subdivided into early responders (white bars) and late responders (black bars). F and G are representative histograms for these early and late responders respectively. Values are means ± SEM. P < 0.05 was considered statistically significant. (*) shows significant differences relative to lean children and a shows significant differences relative to EP-OBIR + children. ObIR-, non-insulin resistant children with obesity; ObIR + , insulin resistant children with obesity; EP-OBIR + , early peak OBIR + children; LP-OBIR + , late peak OBIR + children; DCFDA, 2,7-dichlorofluorescein diacetate