Fig. 5
From: DNMT3A mutation promotes leukemia development through NAM-NAD metabolic reprogramming
Sensitivity of DNMT3A mutant cells to FK866 and the effects of inhibition on NAMPT. A–C Cell viability of OCI-AML3, U937WT and U937MUT after treatment with FK866 for 48 h and the IC50 of each was calculated. D, E Cell apoptosis after shNAMPT or NAMPT inhibition using FK866. F Changes of apoptosis-initiating proteins after NAMPT inhibition using FK866. G, H Cell cycle arrest after knockdown (shNAMPT) or inhibition of NAMPT (FK866). I Changes of cell differentiation-related markers CD11b and CD14 after introduction of the DNMT3A-R882H mutation to U937 cell line. J, K Changes of CD11b and CD14 and cell morphology after knockdown or inhibition of NAMPT. The mean ± SD of minimum n = 3 independent experiments is displayed, representative images shown *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 and ****p ≤ 0.0001 (Student’s T-test)