Fig. 4

Inhibition of NAMPT can induce the depolymerization of Cyclins-CDKs through SIRT6. A–C Co-immunoprecipitation of bindings of Cyclins-CDKs and the corresponding CDKN1A and CDKN1B in OCI-AML3 in which NAMPT was pharmacological or genetic inhibited. D The prediction of interaction between NAMPT and cell-cycle related proteins using the String database. E After NAMPT knockdown, the protein levels of CDKN1A/CDKN1B and Ace-H3K9 were detected by the immunoblotting. F The immunoblotting of the CDKN1A and CDKN1B levels in OCI-AML3 after treatment with UBCS039 or overexpression of SIRT6. G OCI-AML3 cells were genetically or pharmacologically inhibited, and the shNAMPT-OCI-AML3 cells were treated with UBCS039 or overexpressed SIRT6. The immunoblotting of CDKN1A and CDKN1B was then analyzed