Fig. 1

The Dnmt3a R878H mutation causes abnormal NAM metabolism by regulating the expression of Nampt. A UHPLC-HRMS/MS detection results of the serum of the Dnmt3a^R878H/WT mice and the Dnmt3a^WT/WT mice. B KEGG enrichment analysis of the 55 differential metabolites between the two groups. -Log(p) indicates the correlation between the metabolic pathway and the difference between the Dnmt3a^R878H/WT group and the Dnmt3a^WT/WT group. The redder the color of the circle, the greater the correlation. Pathway impact value is based on the pathway topology analysis, indicating the importance of the pathway for this difference to occur. The larger the circle, the greater the importance. C Levels of NAM and 1-methylnicotinamide in serum of the two groups. D Levels of 1-methylnicotinamide and Adenosine 3'-monophosphate in Gr1⁺ cells of the two groups. E GO enrichment analysis of the differential genes in Gr1⁺ cells of the Dnmt3a^R878H/WT mice vs. the Dnmt3a^WT/WT mice. F, G The Nampt transcriptional and translational levels of Gr1⁺ cells in the Dnmt3a^R878H/WT and Dnmt3a^WT/WT mice. H MeDIP-seq analysis of the Nampt gene showed a local hypomethylation pattern in the gene body region of Gr1⁺ cells from the Dnmt3a^R878H/WT mice compared with the Dnmt3a^WT/WT controls. The mean ± SD of minimum n = 3 independent experiments is displayed, representative images shown *p ≤ 0.05, **p ≤ 0.01 (Student’s T-test)