Fig. 6

Uchl3 deficiency lessens BBN-induced bladder tumorigenesis in mice. A Schematic overview showing the strategy of Uchl3 knockout using the CRISPR–Cas9 approach. B Representative image of electrophoresis of Uchl3 in bladder tissues from Uchl3^fl/fl and Uchl3^fl/fl;Upk2-Cre mice. C The protein levels of Uchl3 in bladder tissues from Uchl3^fl/fl and Uchl3^fl/fl;Upk2-Cre mice were detected by Western blotting. D Representative image of Uchl3^fl/fl and Uchl3^fl/fl;Upk2-Cre mice. E The weights of Uchl3^fl/fl and Uchl3^fl/fl;Upk2-Cre mice were measured every 3 days. F Schematic overview showing BBN administration during the experiment to establish the bladder cancer model. G Representative images of BBN-induced bladders removed from Uchl3^fl/fl and Uchl3^fl/fl;Upk2-Cre mice after sacrifice. H Bladder weight of Uchl3^fl/fl and Uchl3^fl/fl;Upk2-Cre mice after the mice were sacrificed. I Statistical analysis of the pathological examination data of bladder tissues from Uchl3^fl/fl and Uchl3^fl/fl;Upk2-Cre mice. J Immunohistochemical analysis of Axin2, Cyclin D1, Lef1, C-Jun and Uchl3 in bladder tissues from Uchl3^fl/fl and Uchl3^fl/fl;Upk2-Cre mice. K Overall survival of Uchl3^fl/fl and Uchl3^fl/fl;Upk2-Cre mice in the BBN-induced bladder cancer model