Fig. 2

Depletion of UCHL3 causes proliferative and migratory defects in bladder cancer cells. A UCHL3-deficient cells were assessed by Western blotting. B Colony formation assays. C A CCK-8 assay was conducted to examine the growth and proliferation ability of UCHL3-deficient cells and control cell lines. D Soft agar assays were performed to reveal the anchorage-independent growth ability of UCHL3-deficient cells and control cell lines. E Migration ability of UCHL3-deficient cells and control cell lines was measured via Transwell migration assay. *P < 0.05; **P < 0.001; ***P < 0.001 compared with controls