Fig. 1
Overexpressing UCHL3 boosts the proliferative and migratory potential of bladder cancer cells. A The expression of Flag-UCHL3 and Flag-UCHL3C94A was assessed by Western blotting. B Colony formation assays demonstrated the proliferation ability of Flag-UCHL3-overexpressing, Flag-UCHL3C94A-overexpressing and control EJ cells. The number of clones was counted and plotted. C Soft agar assays were performed to reveal the anchorage-independent growth ability of the Flag-UCHL3-overexpressing, Flag-UCHL3C94A-overexpressing and control EJ cells. The colony numbers are expressed as the means ± standard errors of triplicate assays. D A CCK-8 assay was conducted to examine the growth and proliferation ability of Flag-UCHL3-overexpressing, Flag-UCHL3C94A-overexpressing and control EJ cells. E Transwell migration assay. The migration ability of Flag-UCHL3-overexpressing, Flag-UCHL3C94A-overexpressing and control EJ cells was measured. *P < 0.05; **P < 0.001; ***P < 0.001 compared with controls