Fig. 4
From: The role of TET2-mediated ROBO4 hypomethylation in the development of diabetic retinopathy
TET2 shRNA alleviates the hypoglycemia-induced abnormalities of human retinal endothelial cell (HRECs) permeability, migration, and tube formation. HRECs were divided into four experimental groups as described in Fig. 3. a The monolayer permeability of HRECs was examined by a transendothelial electrical resistance (TEER) assay. Values were computed by subtracting the resistance (Ω) of the filter alone to each condition and multiplying the result by the total surface of one well (4.67 cm2). b HREC permeability was analyzed by detecting the leakage of FITC-dextran across cells in monolayer culture. c Representative photomicrographs show the migrated cells in Transwell assays. d Quantitative analysis of the migrated cells. e Representative photomicrographs show Matrigel tube formation of HRECs. f, g Quantitative analysis of the number of capillary-like branches and the total branch length. Data represent the mean ± standard deviation. *p < 0.05, **p < 0.01, ***p < 0.001, n = 5 per group