Fig. 2

TRIM22 inhibition enhances melanoma progression in vitro and in vivo. A The RT-qPCR and western blot were used to detect the TRIM22 knockdown (KD) expression levels in M14 cells. B The western blot and RT-qPCR assays were used to confirm the efficiency of TRIM22 overexpression in 501Mel and A2058 cells. C The effect of TRIM22 inhibition or overexpression on melanoma cellular apoptosis was analyzed and quantified by flow cytometric assay. D Cell growth rates of M14 or A375 were enhanced by TRIM22 deficiency. E CCK-8 assays were used to confirm that cell growth rates of 501Mel and A2058 were suppressed in response to ectopic expression of TRIM22. F The colony formation assays were conducted in the indicated groups of A375 or 501Mel cells. G The sphere formation assays were performed in the indicated groups of A375 or 501Mel cells. The sphere numbers in each group were counted and compared. H The wound healing (WH) assays were performed in the indicated groups of A375 or 501Mel cells. I The invasion assays with quantified data were shown in the indicated groups of A375 or 501Mel cells. J The effects of TRIM22 knockdown on subcutaneous tumors were investigated in vivo assays. The quantified tumor volume curve was shown on the right. * P < 0.05, ** P < 0.01, *** P < 0.001