Fig. 9
From: Targeting RNA-binding protein HuR to inhibit the progression of renal tubular fibrosis
TGFß1 induces cytoplasmic translocation of HuR in cultured HK2 cells, which is inhibited by KH3. a, b mRNA expression (a) and total cellular protein production of HuR (b) in cultured HK2 cells. *P < 0.05, vs. cells without treatment. c Representative photomicrographs of cellular immunofluorescent staining for HuR (red) and DAPI (blue) (×400 magnification) from the un-treated HK2 cells (HK2), or HK2 cells treated with KH3 (HK2+KH3) or TGFß1 (KH2+TGFß1) alone or TGFß1 plus KH3 (HK2+TGFß1+KH3). Arrows indicate cytoplasmic staining of HuR. d Representative Western blots illustrating cytoplasmic and nucleus HuR and GAPDH and histone protein expression in untreated and treated HK2 cells. e. The graphs summarize the results of band density measurements for the ratio of cytoplasmic HuR over nucleus HuR. The ratio values are expressed relative to untreated cells, which was set at unity. *P < 0.05, vs. untreated cells; #P < 0.05, vs. TGFß1 alone treated cells