Fig. 2
From: Targeting RNA-binding protein HuR to inhibit the progression of renal tubular fibrosis
Treatment with KH3 reduces renal tubulointerstitial HuR staining and protein expression in the ischemia–reperfusion (IR)-injured mouse kidney. a Representative photomicrographs of renal immunofluorescent staining for HuR (red), DAPI (blue) and wheat germ agglutinin (WGA) (green) (×200 magnification) from the un-injured contralateral kidney (CTL) and IR-injured kidney treated without (IR) or with KH3 (CTL+KH3, IR+KH3). Cytoplasm staining for HuR was able to be seen in IR-injured kidneys as arrows indicated. b Representative Western blots illustrated protein expression of HuR and ß-actin in the whole kidney tissue (n = 5/each group). c Quantification of the band density is shown on the right of Western blot. Protein values are expressed relative to normal control, which was set at unity. KH3 treatment resulted in a significant reduction in renal tubular and tubulointerstitial HuR staining and protein production levels compared with IR-injured kidneys. *P < 0.05, vs. CTL; #P < 0.05, vs. IR