Fig. 7

JAK2 might be involved in the regulation of MAPK3/1 by GH in aged oocytes. A Representative images of p-JAK2 in young, aged, and GH + aged oocytes (in vivo). Scale bar, 20 μm. B p-JAK2 fluorescence intensity in young (n = 20), aged (n = 18), and GH + aged (n = 19) oocytes. C Representative images of p-JAK2 in young, aged and GH + aged oocytes (in vitro). Scale bar, 20 μm. D The fluorescence intensity of p-JAK2 was measured in young (n = 17), aged (n = 15) and GH + aged (n = 18) oocytes. E Representative images of p- MAPK3/1 in aged, GH, and GH + CEP oocytes. Scale bar, 20 μm. F p-MAPK3/1 fluorescence intensity in aged (n = 18), GH (n = 12), and GH + CEP (n = 20) oocytes. G Representative images of spindle morphology and chromosome alignment in aged, GH, and GH + CEP oocytes. Scale bar, 20 μm. H and I Aberrant spindle and misaligned chromosome rates of aged (n = 27), GH (n = 22), and GH + CEP (n = 28) oocytes. J Representative images of chromosome spreading of euploid and aneuploid oocytes. Scale bar, 10 μm. K Aneuploidy rate in aged (n = 25), GH (n = 23), and GH + CEP (n = 26) oocytes. Scale bar, 50 μm. L Representative images of early embryos developed from aged, GH, and GH + CEP oocytes. Scale bar, 50 μm. M Blastocyst formation rate of aged (n = 25), GH (n = 34), and GH + CEP (n = 8) oocytes. Aged, oocytes from aged mice administered PBS; GH, oocytes from aged mice administered GH; GH + CEP, oocytes from aged mice administered CEP and treated with GH. Data are means ± SEM of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001