Fig. 4
From: Growth hormone reduces aneuploidy and improves oocytes quality by JAK2-MAPK3/1 pathway in aged mice
Effect of GH on the development potential of aged oocytes. A Representative time-lapse images of young, aged, and GH + aged embryos at the indicated stages. 2PN, two pronuclei; tPNF, time to pronuclei fading; t2, t3, t4, t5, and t8, time in hours post-tPNF for the embryo to reach the 2-, 3-, 4-, 5-, and 8-cell stages, respectively; tM, time for compaction; tB, time for the blastocoel to reach greater than or equal to half the volume of the embryo. Scale bar, 50 μm. B Fertilization, 2-, 4-, and 8-cell embryo, morula, and blastocyst rates in young (n = 21), aged (n = 18) and GH + aged (n = 22) groups. C Times for embryos to reach the blastocyst stages in the young (n = 21), aged (n = 18), and GH + aged (n = 22) groups. Data are means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001