Fig. 2
From: Growth hormone reduces aneuploidy and improves oocytes quality by JAK2-MAPK3/1 pathway in aged mice
Effect of GH on mitochondrial function in aged oocytes. A Representative images of the mitochondrial distribution in young, aged, and GH + aged MII oocytes. Scale bar, 20 μm. B The rates of abnormal distribution of mitochondria in young (n = 25), aged (n = 21), and GH + aged (n = 24) oocytes. C Representative images of mitochondrial membrane potential (ΔΨm, staining with JC-1) in young, aged, and GH + aged oocytes (red, high ΔΨm; green, low ΔΨm). Scale bar, 20 μm. D Red-to-green fluorescence intensity ratio in young (n = 21), aged (n = 18), and GH + aged (n = 22) oocytes. E Expression of Mfn1, Nrf2, and Nduf revealed by real-time PCR in young, aged, and GH + aged oocytes. Data are means ± SEM of at least three independent experiments. **p < 0.01, ***p < 0.001, ****p < 0.0001