Fig. 7
From: Flubendazole inhibits PD-1 and suppresses melanoma growth in immunocompetent mice
FLU activates JUN and FOS expression in Jurkat T cells and its suppression of PD-1 expression is rescued by T5224. Western blotting was performed with extracts of Jurkat T cells treated with FLU (10 µM) or vehicle for various times. Membranes were treated with antibodies to A JUN, FOS or β-actin, and B PD-1 or β-actin then with secondary antibodies for detection. C Western blotting was performed with antibodies to PD-1 (pretreated with vehicle or 10 µM T5224 for 2 h) then treated with FLU (10 µM) for a further 24 h. Densitometry was performed using NIH Image J. Error bars represent SEM. Data is representative of 3 biologically-independent experiments