Fig. 2
From: Flubendazole inhibits PD-1 and suppresses melanoma growth in immunocompetent mice
FLU inhibits PD-1 levels without affecting PD-L1 and stimulates CD3+ cell accumulation. Immunohistochemical analysis was performed on Day17 B16F10 tumors from vehicle or FLU-treated mice. Quantification and representative photomicrographs of immunostaining for A peritumoral, B intratumoral PD-1+, C peritumoral skin CD3+ and D intratumoral PD-L1+. IOD and tissue area were assessed using Image-Pro Plus® and mean of IOD/µm2 was determined. Representative immunohistochemical staining is shown (right). Data was analyzed by t-test or Mann Whitney test (n = 5–6 mice/group) (left). Error bars represent SEM. S denotes skin, PT denotes peritumoral area, T denotes tumor, IT denotes intratumoral. Arrows indicate positive staining. Dashed lines separate PT and T