Fig. 1

FLU suppresses melanoma growth in immunocompetent mice. A Schedule of mouse treatments. B C57BL/6J mice bearing s.c. B16F10 tumors were administered with FLU or vehicle (200 mg/kg or 20 ml/kg, respectively, i.p.) once daily on a 5 days-on/2 days-off schedule. Treatment commenced on Day 7. Tissues were collected for further analysis on Day 17. Data represent mean ± SEM. n = 6 mice/group. Statistical significance was assessed by t test. ***p < 0.001. C Tumor growth rates were determined by calculating the natural log (ln) of individual tumor volumes on Days 15–17 and calculating slopes in MS Excel. Data represent mean ± SEM. n = 5–6 mice/group. Statistical significance was assessed by Mann Whitney test. D Kaplan–Meier survival analysis was performed with a tumor size limit of 500 mm³ as previously described [51]. n = 6 mice/group. Statistical significance was assessed by log-rank (Mantel-Cox) test