Fig. 5

Olaparib combined with ML216 has a better effect in enhancing total DNA damage. A–D IF staining showing the expression of γH2AX and 53BP1 in PC3 cells treated with ML216 (8 μM) and olaparib (50 μM), ML216 or olaparib monotherapy, or DMSO for 48 h. Scale bars: 25 μm. E Comet assay of PC3 cells treated with ML216 (8 μM) and olaparib (50 μM), ML216 or olaparib monotherapy, or DMSO for 48 h.The olive tail moment was used as an index and was statistically analyzed. Scale bars: 20 μm. F–G Western blot analysis of the expression of proteins involved in the HR and NHEJ pathways in PC3 cells treated with ML216 (8 μM) and olaparib (50 μM), ML216 or olaparib monotherapy, or DMSO for 48 h. Scale bars: 20 μm. Shown are the means ± SD from 3 experiments (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, and “ns” indicated no significant difference)