Fig. 6

Nrf2 influenced anoikis in HeLa and SiHa cells. A The protein levels of Nrf2, HO-1, NQO1, GCLC and GCLM in HeLa and SiHa cells in ATT and SUS were measured. B Quantitative analysis of proteins. C The numbers of surviving cells in the HeLa sg-Ctrl group and HeLa sg-Nrf2 group were counted under different conditions. D The apoptotic percentage of HeLa sg-Ctrl and sg-Nrf2 cells in ATT and SUS through flow cytometry assays. E Testing the expression of Bax, Bcl2, FAK and pro-Caspase-3 in Nrf2 knockout HeLa cells and control cells in ATT and SUS by western blot. F Detection of the expression of Bax, Bcl2, FAK and pro-Caspase-3 in Nrf2-overexpressing cells in the SUS state. * P < 0.05, ** P < 0.01 (n = 3)