Fig. 3

OPN is expressed in macrophages of fibrotic lung tissue. A Confocal microscopy showing F4/80 (green), OPN (red), and DNA dye DAPI (cyan) in lung tissue section of IPF and mice treated with bleomycin and matched group, showing the co-localization of OPN and macrophages. Bars, 50 µm. Co-location cells are indicated by yellow arrows. B Confocal microscopy showing OPN (green), E-cadherin (red), and DNA dye DAPI (cyan) in lung tissue section of IPF and mice treated with bleomycin and matched group, showing the co-localization of OPN and epithelial cells. Bars, 50 µm. C Confocal microscopy showing α-SMA (green), OPN (red), and DNA dye DAPI (cyan) in lung tissue section of IPF and mice treated with bleomycin and matched group, showing the co-localization of OPN and fibroblasts. Bars, 50 µm. D Confocal microscopy showing ABCG2 (green), OPN (red), and DNA dye DAPI (cyan) in lung tissue section of IPF and mice treated with bleomycin and matched group, showing the co-localization of OPN and mesenchymal stem cells. Bars, 50 µm