Fig. 2

OPN expression increases in the lung of bleomycin-treated mice. A–E Bleomycin (5 mg/kg body weight) or saline were administered daily in mice (n≧4), and parameters analyzed on day 7, 14, 21 and 28. A Representative H&E, Masson and IHC staining of mouse lung tissue. Scale bar, 100 μm. Each group was given 5 different visual fields and the positive areas were quantified by average optical density value (n = 3), ****P < 0.0001, ns denotes no significance compared with day 7 group; means + SD. B Immunoblots of OPN, collagen and α-SMA of mouse lung tissue. C QPCR analysis of OPN gene (spp1) expression in the lung of bleomycin-treated mice. 18S was used as the reference gene, *P < 0.05; means + SD. D Concentration of OPN in peripheral blood determined by ELISA, ****P < 0.0001; means + SD. E Concentration of OPN in bronchoalveolar lavage fluid (BALF) determined by ELISA, *P < 0.05, **P < 0.01, ***P < 0.001; means + SD