Fig. 6
![Fig. 6](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12967-023-04275-4/MediaObjects/12967_2023_4275_Fig6_HTML.png)
Calpastatin/Calpain proteolysis system influences cell migration velocity and F-actin behavior in Fam20c-deficient cells. A Gene expression of Calpastatin, Calpain 1, and Calpain 2 in OB Fam20cKO and OB Fam20cKO treated with CaCl2. *P < 0.05, **P < 0.01, ****P < 0.0001. B Western blot analysis of Calpastatin, Calpain 1, and Calpain 2 expression in OB Fam20cKO and OB Fam20cKO treated with CaCl2. GAPDH served as an internal control. C Gene expression of Calpastatin, Calpain 1, and Calpain 2 in OB Fam20cf/f, OB Fam20cKO, and OB Fam20cKO treated with different concentrations of CaCl2. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, NS: no significance. D Phos-Tag sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) (P-tag) demonstrates effects of CaCl2 on Calpastatin phosphorylation levels in OB Fam20cf/f, OB Fam20cKO, and OB Fam20cKO treated with CaCl2. Tubulin served as an internal control. E Casein zymography profiles of Calpain 1 and Calpain 2 in OB Fam20cf/f, OB Fam20cKO, and OB Fam20cKO treated with CaCl2. F Wounding healing assays of OB Fam20cf/f, OB Fam20cKO, and OB Fam20cKO treated with CaCl2, images were acquired 0 h and 24 h after the scratch. Scale bar = 100 μm. G Gene expression of Rac1, RhoA, and Cdc42 in OB Fam20cKO and OB Fam20cKO treated with CaCl2. *P < 0.05, **P < 0.01. H Western blot analysis of Cdc42 expression in OB Fam20cf/f, OB Fam20cKO and OB Fam20cKO treated with CaCl2. GAPDH served as an internal control