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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Fam20c regulates the calpain proteolysis system through phosphorylating Calpasatatin to maintain cell homeostasis

Fig. 6

Calpastatin/Calpain proteolysis system influences cell migration velocity and F-actin behavior in Fam20c-deficient cells. A Gene expression of Calpastatin, Calpain 1, and Calpain 2 in OB Fam20cKO and OB Fam20cKO treated with CaCl2. *P < 0.05, **P < 0.01, ****P < 0.0001. B Western blot analysis of Calpastatin, Calpain 1, and Calpain 2 expression in OB Fam20cKO and OB Fam20cKO treated with CaCl2. GAPDH served as an internal control. C Gene expression of Calpastatin, Calpain 1, and Calpain 2 in OB Fam20cf/f, OB Fam20cKO, and OB Fam20cKO treated with different concentrations of CaCl2. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, NS: no significance. D Phos-Tag sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) (P-tag) demonstrates effects of CaCl2 on Calpastatin phosphorylation levels in OB Fam20cf/f, OB Fam20cKO, and OB Fam20cKO treated with CaCl2. Tubulin served as an internal control. E Casein zymography profiles of Calpain 1 and Calpain 2 in OB Fam20cf/f, OB Fam20cKO, and OB Fam20cKO treated with CaCl2. F Wounding healing assays of OB Fam20cf/f, OB Fam20cKO, and OB Fam20cKO treated with CaCl2, images were acquired 0 h and 24 h after the scratch. Scale bar = 100 μm. G Gene expression of Rac1, RhoA, and Cdc42 in OB Fam20cKO and OB Fam20cKO treated with CaCl2. *P < 0.05, **P < 0.01. H Western blot analysis of Cdc42 expression in OB Fam20cf/f, OB Fam20cKO and OB Fam20cKO treated with CaCl2. GAPDH served as an internal control

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