Fig. 8

DRP1 knockdown reduced M1 polarization mediated foam cell formation by inhibiting mito-ROS/NLRP3 activation. A The protein level of DRP1 detected by western blot. The relative protein expression levels of (B) DRP1/β-actin in RAW264.7 cells. C, D ORO staining to detect lipid deposition in macrophages (scale bar: 100 μm. 200 × magnification). qRT-PCR to detect the mRNA expression of M1 polarization markers, including (E) iNOS, (F) IL-6 and M2 markers, including (G) CD206, and (H) IL-10. I, J mito-ROS detected by mito-SOX (scale bar: 25 μm. 200 × magnification). (K, L) The SOD activity and level of MDA detected by corresponding kits. M The protein level of NLRP3, pro-caspase-1, and cleaved-caspase-1 detected by western blot. The relative protein expression levels of (N) NLRP3/β-actin (O) pro-caspase-1/β-actin and (P) cleaved-caspase-1/β-actin in RAW264.7 cells. Three independent replications were performed. * p < 0.05, ** p < 0.01, *** p < 0.001, and ns p > 0.05