Fig. 6

YBX1 is involved in CRC cell proliferation and metastasis. A, B Images showing signal intensities and the corresponding IHC scores. A total of 195 images were scored. Scale bar: 50 μm (down). C, D YBX1 expression in CRC patient tumors of different stages (C from TAM and D from TCGA datasets; Nature 2012). E WB analysis of YBX1 expression in HCT116 cells transduced with the YBX1 overexpression vector and control vector. F, G Colony formation and Transwell assays (scale bars: 100 μm) using HCT116 cells showed the effects of YBX1 on cell proliferation and metastasis. The data are presented as the means ± SDs. Statistical significance was assessed using one-way ANOVA followed by Tukey’s test for multiple comparisons. All experiments were performed with at least three biological duplicates (n = 3) for each group. *P < 0.05, ***P < 0.001