Fig. 2

RP11-296E3.2 regulates CRC cell proliferation and metastasis in vitro. A, B GO enrichment analysis was performed for 132 SDEGs between RP11-296E3.2 overexpressed cells and Vector cells (n = 3 samples per group), and 99 SDEGs overlapping between two sh-RP11-296E3.2 and sh-Con cells (n = 3 samples per group)by online DAVID analysis (https://david.ncifcrf.gov/summary.jsp). C, D RTCA was performed to evaluate the proliferation of HCT116 and RKO cells transduced with the sh-Con and two sh-RP11-296E3.2 vectors (n = 6 per group). E, F Colony formation assay showing the effect of RP11-296E3.2 on the growth of RP11-296E3.2-silenced HCT116 and RKO cells relative to the corresponding sh-Con cells. G, H Representative images of Transwell assays using HCT116 and RKO cells showing cell metastasis and invasion after knockdown of RP11-296E3.2 and a histogram showing the counts of migrated cells. Scale bars: 100 μm. The data are presented as the means ± SDs. Statistical significance was assessed using one-way ANOVA followed by Tukey’s test for multiple comparisons. All experiments were performed with at least three biological duplicates (n = 3) for each group. *P < 0.05, **P < 0.01, ***P < 0.001