Fig. 1
Basic information and bioinformatics analyses of RP11-296E3.2. A Schematic illustration of previous clinical studies of RP11-296E3.2 in CRC metastasis. B The UCSC Genome Bioinformatics Site (http://genome.ucsc.edu/) showed the location of RP11-296E3.2. C Coding Potential Calculator 2 (http://cpc2.gao-lab.org/) showed the protein-coding ability of RP11-296E3.2. D The 5′ and 3′ sequences of RP11-296E3.2 were confirmed by RACE and MS. E Confocal images showing the RP11-296E3.2 distribution in HCT116 and RKO cells; U6 was used as the nuclear marker. Scale bars: 50 μm. F, G qRT‒PCR analysis of RP11-296E3.2 expression in RP11-296E3.2-transduced, RP11-296E3.2-silenced and control cells as indicated. The data are presented as the means ± SDs. Statistical significance was assessed using one-way ANOVA followed by Tukey’s test for multiple comparisons. All experiments were performed with at least three biological duplicates (n = 3) for each group. *P < 0.05, **P < 0.01, ***P < 0.001. ****P < 0.0001. H 3D PCA showed the differences among the three replicates per group in the RNA-seq results. I, J Heatmap and volcano plot of SDEGs between RP11-296E3.2-overexpressing and Vector cells. Light red (upregulated) and green (downregulated): genes with |log2FC| > 1 and Padj < 0.05. K, L Heatmap and volcano plot of SDEGs between sh-RP11-296E3.2 and sh-Con cells. Light red (upregulated) and green (downregulated): genes with |log2FC| > 1 and Padj < 0.05