Fig. 4
From: Mitochondrial DNA induces nucleus pulposus cell pyroptosis via the TLR9-NF-κB-NLRP3 axis
OS induced mPTP opening and cytosolic mtDNA over-release in NPCs. Human NPCs was cultured with 0, 100, and 200 µM H2O2 for 24 h. a, c Fluorescence images of Calcein AM/Co2+ staining and quantitative analysis of fluorescence intensity for the opening of mPTPs in the NPCs. b, d Scatter plots of JC-1 staining and quantitative analysis of MMP in NPCs. e, f Quantitative analysis of fluorescence intensity and fluorescence images of Mito-tracker for mitochondrial activity in the NPCs. g Fluorescence images of dsDNA (green) in the NPCs. h TEM images of the NPCs. i, j Agar gelatin electrophoresis images of mtDNA (ND1 and ND2) in the cytoplasmic or total lysates in NPCs. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001