Fig. 7

Sequencing data validation and diversity analysis of gut microbiota. Sobs rarefaction curve (A) and Shannon rarefaction curve (B) were drawn by the OTU number sampled. Indices of richness (Ca,b), diversity (Da,b), and evenness (Ea,b) estimators of the T1D, T1D + NaB, and control groups were used for the α-diversity analysis. 2D (F) and 3D (G) Principal component analysis plots comparing sample distribution in the three groups. H Hierarchical clustering at the OTU level. Data are presented as the mean ± SEM. *p < 0.05, n = 5