Fig. 5

Effects of sevoflurane on lung epithelial barrier function of mouse lung epithelial (MLE-12) cell monolayer, treated or not with RAGE antagonist peptide (RAP). a Electrical resistance over 24 h of a monolayer of MLE-12 cells was measured at a frequency at 4000 Hz by electric cell-substrate impedance sensing (ECIS) in untreated cells (Medium) or in cells treated for 24 h with cytomix alone (Cyto), sevoflurane alone (Sevo), cytomix and sevoflurane (Cyto + Sevo), cytomix and RAP (Cyto + RAP) or with cytomix, RAP, and sevoflurane (Cyto + RAP + Sevo). Results are shown as medians with interquartile ranges (n = 35–40 per group and per timepoint). Two-way ANOVA test was performed, with post-hoc comparisons if ANOVA results showed significance (compared to the Medium group: ****p < 10^–4; compared to the Cyto + Sevo group: ++p < 0.01). b Medium level of Chemokine C-X-C motif ligand-1(CXCL-1), c Interleukin 6(IL-6) and d Tumor necrosis factor alpha (TNF-α) at 6 h in identical conditions. e) western blots of ZO-1 and E-cadherin at 6 h in identical conditions. f) Protein expression levels were quantified and standardized by GAPDH protein level and expressed as ratios to those in the Medium group. Results of b–f are shown as medians with interquartile ranges. One-way ANOVA was performed, with post-hoc comparisons if ANOVA results showed significance (compared to the Medium group: *p < 0.05; ****p < 10^–4; compared to the Cyto group: ^#p < 0.05; ^##p < 0.01)