Fig. 4
Folate supplementation effectively reduces silica-induced pulmonary fibrosis and oxidative stress. A Schematic diagram of folic acid (FA) supplementation in silica-induced pulmonary fibrosis mouse model. B, C Paraffin-embedded sections of lung tissue was stained with H&E and Masson staining, respectively. The areas marked with numbers are further enlarged. Scale bar = 100 μm. D Paraffin-embedded sections of lung tissue were analyzed by immunohistochemical staining with indicated antibodies. Scale bar = 100 μm. E–J Gene expression levels of different lung tissues were determined by RT-qPCR. Results are expressed as mean ± SD, n = 4 mice/group, * represents P < 0.05. K, L Concentration analysis of H2O2 and MDA of different lung tissues. Results are expressed as mean ± SD, n = 4 mice/group, * represents P < 0.05