Fig. 3
Folate supplementation suppresses TGF-β induced oxidative stress during myofibroblast differentiation. A–E Gene expression levels in cells treated with 5-mTHF and TGF-β1 as indicated were determined by RT-qPCR. Results are expressed as mean ± SD, n = 3, * represents P < 0.05. F Lysates from cells treated with 5-mTHF and TGF-β1 as indicated were analyzed by western blotting. G, H Concentration analysis of H2O2 and MDA in cells treated with 5-mTHF and TGF-β1 as indicated. Results are expressed as mean ± SD, n = 3, * represents P < 0.05. I–L Flow cytometry analysis of total intracellular ROS (DCF) and mitochondrial ROS (MitoSOX Red) in cells treated with 5-mTHF and TGF-β1 as indicated. Results are expressed as mean ± SD, n = 3, * represents P < 0.05. M–P Flow cytometry analysis of total intracellular ROS (DCF) and mitochondrial ROS (MitoSOX Red) in cells treated with siRNA and TGF-β1 as indicated. Results are expressed as mean ± SD, n = 3, * represents P < 0.05