Fig. 2

MTHFD2 negatively regulates TGF-β induced myofibroblast differentiation. A–D The mRNA levels of MTHFD2 and myofibroblast markers (FN1, COL1A1, α-SMA) in cells treated with siRNA and TGF-β1 as indicated for 48 h were determined by RT-qPCR. Results are expressed as mean ± SD, n = 3, * represents P < 0.05. E Lysates from cells treated with siRNA and TGF-β1 as indicated for 48 h were analyzed by western blotting with indicated antibodies. F–I The mRNA levels of MTHFD2 and myofibroblast markers (FN1, COL1A1, α-SMA) in cells transfected with indicated plasmids were determined by RT-qPCR following TGF-β1 treatment for 48 h. Results are expressed as mean ± SD, n = 3, * represents P < 0.05. J Lysates from cells transfected with indicated plasmids were analyzed by western blotting with various antibodies following TGF-β1 treatment for 48 h