Fig. 6

CircNFATC3 binds to IGF2BP3 to regulate the expression of CCND1 in GC. A Potential target screening strategy of the circNFATC3-IGF2BP3 axis. B-D The protein levels of CCND1 in SGC7901 and BGC823 cells following IGF2BP3 overexpression (B) or knockdown (C) or circNFATC3 knockdown (D). E Protein expression of CCND1 in SGC7901 and BGC823 cells transfected with NC, si-circNFATC3 or si-circNFATC3 combined with IGF2BP3 overexpression. F-G RIP analyses of CCND1 enrichment pull-downs by IGF2BP3 in SGC7901 and BGC823 cells following IGF2BP3 (F) and circNFATC3 (G) knockdowns. H RIP analysis of CCND1 enrichment pulled down by GFP in SGC7901 and BGC823 cells transfected with full-length or IGF2BP3 truncations. I-K Stability of CCND1 mRNA at indicated times following IGF2BP3 overexpression (I) or knockdown (J) or circNFATC3 knockdown (K) in SGC7901and BGC823 cells. The Student’s t test was used to calculate p-values. ns, not significant, *P < 0.05, ***P < 0.001