Fig. 3

Knockdown of circNFATC3 inhibits the proliferation of GC cells in vitro and in vivo. A-C The proliferation of GC cells transfected with circNFATC3 siRNAs and evaluated by CCK-8 (A), plate colony formation (B), and EdU assays (C). D-F Tumor images (D), growth curves (E), and tumor weights (F) were obtained from xenograft tumor derived from HGC-27 cells which treated with cholesterol modified siRNAs targeting circNFATC3. G Relative expression of circNFATC3 in xenografted tumor tissues. H Representative images of Ki-67 expression evaluated by IHC in xenografted tumor tissues. Three different visual fields were randomly selected for each section for staining scoring. The Student’s t test (A-C, E–G) and Mann–Whitney U test (H) were used to calculate p-values. ** P < 0.01, and *** P < 0.001