Table 1 Sequences of PCR primers
Gene symbol | Accession number | Forward primer (5′–3′) | Reverse primer (5′–3′) | Expected amplicon size (base pair) |
|---|---|---|---|---|
β-actin | NM_007393 | TATGCTCTCCCTCACGCCATCC | GTCACGCACGATTTCCCTCTCAG | 129 |
Nephrin | NM_019459 | CTCCACGGTTAGCACAGCAGAAG | GCTTGGCGATATGACACCTCTTCC | 102 |
WT1 | NM_144783 | CAGTGAAATGGACAGAAGGGCAGAG | ATACACGCCGCACATCCTGAATG | 141 |
PPARA | NM_011144 | CTTCACGATGCTGTCCTCCTTGATG | GATGTCACAGAACGGCTTCCTCAG | 112 |
ESR1 | NM_007956 | CCTGGCTGGAGATTCTGATGATTGG | TCCACCATGCCTTCCACACATTTAC | 124 |
SLC2A1 | NM_011400 | GATGAAAGAAGAGGGTCGGCAGATG | CAGCACCACAGCGATGAGGATG | 106 |
SHBG | NM_011367 | CTTCTGCTTCCTTCTGCCTGAGTG | CTAGTGGGAGGTGCGGGTATCG | 140 |
AR | NM_013476 | GGCAGCAGTGAAGCAGGTAGC | CAGAGCCAGCGGAAAGTTGTAGTAG | 129 |
AKR1B1 | NM_009658 | AAGCCTGAAGATCCGTCTCTCCTG | CGCACTGGTGTCACAGACTTGG | 143 |
PPARG | NM_011146 | GCCAAGGTGCTCCAGAAGATGAC | GTGAAGGCTCATGTCTGTCTCTGTC | 102 |
IGF1R | NM_010513 | ATGCCAACAAGTTCGTCCACAGAG | TCCGTCTCGTAGATGTCTCGTGTC | 112 |
RELA | NM_009045 | CCAGACCAACAACAACCCCTTCC | AAGCAGAGCCGCACAGCATTC | 81 |
PIK3CA | NM_008839 | GCACAAGAGTACACCAAGACCAGAG | GCATTCCAGAGCCAAGCATCATTG | 130 |
MMP9 | NM_013599 | CGCCACCACAGCCAACTATGAC | CTGCTTGCCCAGGAAGACGAAG | 130 |
AKT1 | NM_009652 | TCAGGATGTGGATCAGCGAGAGTC | AGGCAGCGGATGATAAAGGTGTTG | 108 |
INSR | NM_010568 | ATCCGCCGCTCCTATGCTCTG | GAGTTGCCTCAGGTTCTGGTTGTC | 126 |
MMP2 | NM_008610 | ACCATGCGGAAGCCAAGATGTG | AGGGTCCAGGTCAGGTGTGTAAC | 126 |
TTR | NM_013697 | ACACTTGGCATTTCCCCGTTCC | GCGATGGTGTAGTGGCGATGG | 83 |