Fig. 2
Differential proteomic signature of the four ocular immune diseases. A Volcano plots indicating DEPs between diseased patients and HCs. B Principal component analysis results showing the expression patterns of HCs and diseased patients based on the DEPs between diseased patients and HCs. C Bubble plots describing the GSEA results of sEVs, lEVs, and plasma. D Score plots showing the PLS-DA model using proteins in sEVs (R2 = 0.965, Q2 = 0.781, p-value < 0.05, 3 components), lEVs (R2 = 0.962, Q2 = 0.653, p-value < 0.05, 4 components) and plasma (R2 = 0.940, Q2 = 0.807, p-value < 0.05, 2 components); The number of PLS-DA components was determined according to the highest Q2 value. E Rose-Chart showing the DEPs in the individual components of the four diseases. F Venn diagrams showing the overlap of significantly upregulated DEPs in the four diseases among sEVs, lEVs, and plasma, and the overlap of significantly upregulated DEPs in the three components among the four diseases. G Heatmaps displaying the score of canonical intracellular pathways by GSVA of the four diseases and HC. Red represents relative activation, while blue represents relative inhibition. AS ankylosing spondylitis-related acute anterior uveitis, BD Behcet's disease uveitis, SCL posterior scleritis, VKH Vogt-Koyanagi-Harada syndrome, HC healthy control, DEP differentially expressed protein