Fig. 2

FSCN1 is highly expressed and colocalized in pathological choroidal neovascularization A Clustering is performed using resolution = 0.1 and visualized using UMAP algorithm from the publicly available scRNA-seq data of AMD patients (GEO: #GSE135922) through the Seruat package. B Cross validation of the identified EC marker genes (CDH5 and PECAM1) and FSCN1 from the publicly available scRNA-seq data (GEO: #GSE135922) of AMD patients by density plot through the Nebulosa package. The right side of the plot shows the scale of expression density. C Quantitative reverse‐transcription polymerase chain reactions (qRT–PCRs) were performed to detect FSCN1 levels in primary choroidal endothelial cells of mice at 7 days after laser-induced choroidal neovascularization (CNV). Results are presented as mean ± SEM, statistical analyses were performed using two-tailed student's t-test. (n = 4 mice per group, data pooled from 4 independent experiments). D, E Colocalization of FSCN1 and pathological choroidal neovascularization (IsoB4: red; FSCN1: green). Scale bar: 200 μm. Quantification of Manders' coefficient (right panel). Results are presented as mean ± SEM. (n = 4 independent experiments). (M1: red pixels overlapping green pixels, M2: green pixels overlapping red pixels)