Fig. 7

Immune cell infiltration of MOC1 and MOC1-HPV tumors. A, B Representative images of immunofluorescently stained frozen sections of the tumor core (A) or tumor edge (B). T lymphocytes were marked with CD4 or CD8 (orange), macrophages with F4/80 (green), blood vessels with CD31 (magenta), and cell nuclei with Hoechst 33,342 (blue). Scale bar: 100 µm. C, D Numbers of CD4 T lymphocytes per mm² of tumor core (C) and tumor edge (D). E, F Numbers of CD8 T lymphocytes per mm² of tumor core (E) and edge (F). G, H CD4/CD8 ratio in the tumor core (G) and tumor edge (H). I, J Percentage of F4/80-positive area in the tumor core (I) and tumor edge (J). Bar plots present the mean ± SD. n = 4. ns not significant, ordinary one-way ANOVA