Fig. 6

Tumor microenvironment of MOC1 and MOC1-HPV tumors. A Representative images of immunofluorescently stained frozen sections of the tumor core. Proliferating cells are marked with EdU (Green), hypoxic regions with EF5 (Orange), blood vessels with CD31 (Magenta), and cell nuclei with Hoechst 33342 (Blue). Scale bar: 200 µm. B–E The fluorescent signal was quantified and expressed as the percentage of proliferating cells (B), percentage of hypoxic area (C), percentage of tumor blood vessel area (D), and average vessel size (E). Bar plots present the mean ± SD. The black line in the dot plot presents the mean. n = 4. ns not significant, **p < 0.01, ***p < 0.001, ****p < 0.0001, ordinary one-way ANOVA (B–D) and nonparametric one-way ANOVA (Kruskal‒Wallis test) (E)