Fig. 5

MOC1-HPV tumors express HPV oncogenes E6 and E7 and show histological features of squamous cell carcinomas. A Expression of the HPV-16 E6 and E7 oncogenes in tumor tissue was determined at a tumor volume of 50 mm³ using qRT‒PCR. Housekeeping genes: GAPDH and β-actin. Presented is the mean ± SD. n = 4–5. B–F Representative images of hematoxylin and eosin-stained paraffin sections of MOC1 (B), MOC1-HPV K1 (C, D), and MOC1-HPV K3 (E, F) tumor cores. Tumors were isolated when they reached volumes of 50–100 mm³. Dotted lines denote necrotic areas, asterisks denote desmoplasia, black denote arrows keratinization, white arrows denote apoptotic cells, red arrows denote cells with large nuclei, and yellow arrows denote cells with a perinuclear halo. Images B–E were acquired using a 10 × objective, and image F was acquired with a 20 × objective. Scale bar: 100 µm. n = 4