Fig. 2
In vitro characterization of MOC1 and MOC1-HPV cell lines. A Representative immunofluorescent images of MOC1 and MOC1-HPV cells after staining of the plasma membrane with WGA (red) and nuclei with Hoechst 33342 (blue). Scale bar: 40 µm. B Two representative cell colonies of MOC1, MOC1-HPV K1, and MOC1-HPV K3 cells six days after seeding. Scale bar: 200 µm. C Average cell diameter of adherent cells. Dot plots present all data with the black line representing the mean. Between 50 and 65 cells of each cell line were analyzed. ****p < 0.0001, ns: not significant, nonparametric one-way ANOVA (Kruskal‒Wallis test). D Cell diameter of trypsinized cells in suspension. In total, up to 11,000 cells of each cell line from 10 individual experiments were analyzed. The bar plot presents the mean of each experiment ± SD. **p < 0.01, ****p < 0.0001, ns not significant, ordinary one-way ANOVA. E Proliferation of MOC1 and MOC1-HPV cell lines. Presented are the mean + SD. n = 3. F Cell front velocity of MOC1 and MOC1-HPV cell lines in the wound-healing assay. Presented are the mean ± SD. ****p < 0.0001, ordinary one-way ANOVA. n = 6. G, H Representative bright-field images of cell migration in the wound-healing assay. Scale bar: 500 µm (G) or 250 µm (H)