Fig. 1

MOC1-HPV monoclonal cell lines express HPV-16 E6 and E7 at the RNA and protein levels. A, B Expression of HPV-16 E6 (A) and E7 (B) at the mRNA level remains stable with cell passaging as determined by qRT‒PCR. Housekeeping genes: GAPDH and β-actin. The data are presented as the mean ± SD. n = 2. C, D Representative images of cells immunofluorescently stained for HPV-16 E6 (C) or HPV-16 E7 (D). Scale bar: 10 µm. E–H Mean fluorescence intensity of E6 (E, F) and E7 (G, H) in the nucleus and cytoplasm. Dot plots present all data with the black line representing the mean. Up to 100 cells of each cell line were analyzed. *p < 0.05, ***p < 0.001, ****p < 0.0001, one-way ANOVA