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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Suicide gene therapy using allogeneic adipose tissue-derived mesenchymal stem cell gene delivery vehicles in recurrent glioblastoma multiforme: a first-in-human, dose-escalation, phase I clinical trial

Fig. 2

HEK-293T, as packaging host, were co-transfected by psPAX2, pMD2.G, and PCDH-TK with the ratio of 2:1:2 and calcium phosphate reagent (A). Proliferation rate of high efficiency transduced MSCs based on fluorescent microscopy after 72 h (B). Flow cytometry assay confirmed the population of GFP + cells (92%) (C). The relative HSV-TK mRNA overexpression level in transduced MSCs and U-251 cells compared to control is demonstrated via bar chart (** p < 0.01)

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Journal of Translational Medicine

ISSN: 1479-5876

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