Fig. 3

Intertumoral metabolic heterogeneity in G3/G4 MB. A Integrated clustering of six (two G3, four G4) human MB single-cell transcriptomes is shown in a two-dimensional UMAP plot. B Cell clusters have been annotated regarding their affiliation to G3 and G4 MB or as non-malignant TME cells using cell counts and different marker gene signatures (Additional file 1: Figs. S7, S8). C, D UMAP plots showing overlaps between G3/G4 MB groups and the metabolic gene signatures obtained from ICGC or MAGIC. E The analysis has been repeated using a second scRNA-seq dataset derived from Riemondy et al., encompassing samples from all four MB groups. Moreover, UMAPs based on single-cell RNA expression of metabolic genes from ccmGDB, also used for bulk RNA analysis, are shown for F MSMB and G RMB cohorts. Annotation of cell types from the TME and metabolic processes highlighted in these metabolic UMAPs has been performed as described in the Additional file 1. ECM = extracellular matrix; PPP = pentose phosphate pathway